Monday, December 2, 2013

Oswald Avery, Colin MacLeod, and Maclyn McCarty expereiment

Oswald Avery, Colin MacLeod, and Maclyn McCarty showed that the substance
causing the transformation of R cells into S cells was DNA.
 In doing these experiments, they first had to develop chemical procedures for isolating almost pure DNA from cells, which had never been done before. When they added DNA isolated from S cells to growing cultures of R cells, they observed transformation: A few cells of type S cells were produced. Although the DNA preparations contained traces of protein and RNA,the transforming activity was not altered by treatments that destroyed either protein or RNA.
 However, treatments that destroyed DNA eliminated the transforming activity .
These experiments implied that the substance responsible for genetic transformation was the DNA of the cell—hence that DNA is the genetic material.
Their experiment is given below

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